For at ændre ”Enhedens navn” og ”Sted og dato”: Klik i menulinjen, vælg ”Indsæt” > ”Sidehoved / Sidefod”. Indføj ”Sted og dato” i feltet for dato og ”Enhedens navn” i Sidefod PAC spectroscopy applied in biochemistry: Bacterial metal ion sensor proteins Lars Hemmingsen ISOLDE workshop 2015 Department of Chemistry, Faculty of Science Giedroc and Arunkumar Dalton Trans. 2007, 3107

Chemical warfare between host and pathogen Edwin Smith papyrus (~1600 BC): Copper to sterilize water and wounds co.uk/ViewItemvi. php?ItemID=Ladies -Copper-Pyjamas-20

Tekst starter uden punktopstilling For at få punkt- opstilling på teksten, brug forøg indrykning For at få venstre- stillet tekst uden punktopstilling, brug formindsk indrykning Overskrift her For at ændre ”Enhedens navn” og ”Sted og dato”: Klik i menulinjen, vælg ”Indsæt” > ”Sidehoved / Sidefod”. Indføj ”Sted og dato” i feltet for dato og ”Enhedens navn” i Sidefod In E. coli Cu I and Ag I is controlled by CueR Changela et al., Science, 2003, 301, 1383; Philips et al. Science, 2015, 349, 877

CueR metal binding loop: How are mono- and divalent metal ions discriminated? Szunyogh et al. accepted, Angew. Chem. Int. Ed. 199m Hg PAC 1 H-NMR Hg II -MBL

CueR metal binding loop: How are mono- and divalent metal ions discriminated? Szunyogh et al. accepted, Angew. Chem. Int. Ed. 1 H-NMR Hg II -MBL 1 H-NMR Ag I -MBL Ag I -MBL Free MBL Contrary to Hg II (and Zn II ), Ag I binding displays either Coordination of only one Cys, or Coordination of a protonated Cys At near neutral pH

The allosteric mechanism: A role for protonated Cys112?

Two co-existing metal site structures in the Cd II biosensor CadC? CdS 4 CdS 3 X Strange 113 Cd NMR resonance in CadC: Not CdS 4 nor CdS 3 X 111m Cd PAC on model systems Tebo et al. Metallomics 2015, 7, 1555

Two co-existing metal site structures in the Cd II biosensor CadC? CdS 4 CdS 3 X Strange 113 Cd NMR resonance in CadC: Not CdS 4 nor CdS 3 X Two co-existing coordination geometries, CdS 3 O and CdS 4, in exchange on the μs time scale coherently accounts for all existing spectroscopic data (UV absorption, PAC, NMR, and EXAFS). Multiple coordination geometries may be a common feature and central to function in biochemical metal ion sensors. 111m Cd PAC on model systems Tebo et al. Metallomics 2015, 7, 1555

Collaborations University of Szeged, Hungary University of Michigan, USA Alison Tebo Vincent L. Pecoraro Karl Joao Monika Magdalena Stavroula Juliana Johnston G.M. Correia Stachura Kowalska Pallada Schell Attila Jancso Béla Gyurcsik, Daniel Zsunyogh H. Zsolokai Flemming H. Niels J. Larsen Christensen Peter W. Thulstrup

Funding