9 th January 2007 – © Alexander Schulz Laboratory for Plant Physiology and Anatomy 1 Nyudviklet nanosensor for fosfat Hong Gu, Iver Jakobsen, Wolf B. Frommer,

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1 9 th January 2007 – © Alexander Schulz Laboratory for Plant Physiology and Anatomy 1 Nyudviklet nanosensor for fosfat Hong Gu, Iver Jakobsen, Wolf B. Frommer, Alexander Schulz

2 9 th January 2007 – © Alexander Schulz 2 Tilgængelighed af uorganisk fosfat (P i ) i jorden organisk P opløst P i <10 µM total P i jorden bundet, mineralsk P Poirier & Bucher 2002, Fig. 1

3 9 th January 2007 – © Alexander Schulz 3 Fosfatoptagelse via rodhår lav fosfat (5µM) høj fosfat (5mM) Poirier & Bucher 2002, Fig. 7

4 9 th January 2007 – © Alexander Schulz 4 Optagelse via hårenes overflade

5 9 th January 2007 – © Alexander Schulz 5 Cellulær fosfatkoncentration P i i jordvand < 10 µM P i i cellerne5-10 mM = 1000 gange akkumulering! aktiv optagelse

6 9 th January 2007 – © Alexander Schulz 6 Fosfatoptagelse sker i forskellige væv Campbell & Reece 2005, Fig. 36,9 Poirier & Bucher 2002, Fig. 16 aktiv optagelse transportvej gennem rodvævet

7 9 th January 2007 – © Alexander Schulz 7 Fosfatfordeling i planten

8 9 th January 2007 – © Alexander Schulz 8 Brug for cellulær P i måling Hvilke celletyper kan optage P i ? Hvad er deres cellulære P i koncentration? Hvordan er den cellulære P i reguleret?

9 9 th January 2007 – © Alexander Schulz 9 Målemetode: nanosensorens farveskift

10 9 th January 2007 – © Alexander Schulz 10 A lav P i B høj P i Ændring af de spektrale egenskaber B A A B

11 9 th January 2007 – © Alexander Schulz 11 Måling in vitro

12 9 th January 2007 – © Alexander Schulz 12 Tre måleområder

13 9 th January 2007 – © Alexander Schulz 13 Målesensorerne er inde i cellerne

14 9 th January 2007 – © Alexander Schulz 14 P i svingning i rodvæv 10mM50mM 100mM ekstern P i

15 9 th January 2007 – © Alexander Schulz 15 Bioimaging gruppe kim kristiansen michael hansen helle martens alexander schulz Reactive oxygen species Nanosensorer Microscope techniques hong gu Short- and longdistance transport Short- and longdistance transport